Coding

Part:BBa_K2486103:Design

Designed by: Tiago Lubiana Alves   Group: iGEM17_USP-Brazil   (2017-10-27)


Anti-malarial single-chain antibody m4B7 (no stop codon)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 573
    Illegal PstI site found at 676
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 573
    Illegal PstI site found at 676
    Illegal NotI site found at 736
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 573
    Illegal XhoI site found at 435
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 573
    Illegal PstI site found at 676
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 573
    Illegal PstI site found at 676
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No stop codon added facilitating fusion of secretion tag.


Source

Derived from mouse monoclonal antibodies. Can be synthesized.

References